Journal: Molecular Medicine Reports

Article Title: Effects of Tanshinone IIA on the modulation of miR-33a and the SREBP-2/Pcsk9 signaling pathway in hyperlipidemic rats

doi: 10.3892/mmr.2016.5133

Figure Lengend Snippet: Tanshinone IIA regulation of miR-33a, ABCA1 and ABCG1 expression in the liver. (A) RT-qPCR measurement of miR-33a expression in rat liver tissue. (B) RT-qPCR and western blotting analyses of ABCA1 and ABCG1 expression in liver tissue. Data are presented as the mean ± standard deviation. * P<0.05, ** P<0.01. miR, microRNA; ABCA1, ATP-binding cassette transporter A1; ABCG1, ATP-binding cassette transporter G1; RT-qPCR, reverse trancription-polymerase chain reaction; CON, control; HLP, high lipid diet-fed rats; TAN, Tanshinone IIA treatment of high lipid diet-fed rats; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.

Article Snippet: The membranes were then incubated overnight at 37°C with rabbit anti-rat SREBP-2, LDL-R, PCSK9, ABCA1 (cat no. bs-1627R), ABCG1 (cat no. bs-1231R) and GAPDH (No. bs-2188R) primary antibodies (all 1:300; BIOSS), and then with the HRP-conjugated goat anti-rabbit secondary antibody (1:200) for 1 h at 37°C.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Standard Deviation, Binding Assay, Polymerase Chain Reaction

Journal: Molecular Medicine Reports

Article Title: Effects of Tanshinone IIA on the modulation of miR-33a and the SREBP-2/Pcsk9 signaling pathway in hyperlipidemic rats

doi: 10.3892/mmr.2016.5133

Figure Lengend Snippet: Tanshinone IIA regulation of SREBP-2, Pcsk9 and LDL-R mRNA and protein expression levels in the liver. (A) Reverse transcription-polymerase chain reaction measurement of SREBP-2, Pcsk9 and LDL-R mRNA expression levels in rat liver tissue. (B) Western blotting analysis of SREBP-2, Pcsk9 and LDL-R protein expression levels in the liver. Data are presented as the mean ± standard deviation. * P<0.05, ** P<0.01. SREBP-2, sterol regulatory element-binding protein 2; Pcsk9, proprotein convertase subtilisin/kexin type 9; LDL-R, low-density lipoprotein receptor; CON, control; HLP, high lipid diet-fed rats; TAN, Tanshinone IIA treatment of high lipid diet-fed rats; GAPDH, .glyceraldehyde 3-phosphate dehydrogenase.

Article Snippet: The membranes were then incubated overnight at 37°C with rabbit anti-rat SREBP-2, LDL-R, PCSK9, ABCA1 (cat no. bs-1627R), ABCG1 (cat no. bs-1231R) and GAPDH (No. bs-2188R) primary antibodies (all 1:300; BIOSS), and then with the HRP-conjugated goat anti-rabbit secondary antibody (1:200) for 1 h at 37°C.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Standard Deviation, Binding Assay

Journal: Experimental and Therapeutic Medicine

Article Title: Effects of norepinephrine on colonic tight junction protein expression during heat stress

doi: 10.3892/etm.2021.9865

Figure Lengend Snippet: Expression levels of Occludin, ZO-1, TRPA1 and PAR-2 in the rat distal colon tissue sections in control and heat exposure groups. The figures (A, B) shown are representative of three different animals. Magnification, x200. Black arrows indicate the musca. Red arrows indicate the myenteric plexus. ZO-1, zonula occludens-1; PAR-2, protease-activated receptor 2; TRPA1, transient receptor potential ankyrin 1 channel; CM, circular muscle; LM, longitudinal muscle.

Article Snippet: The cells were then incubated with primary antibodies against PAR-2 (1:300; cat. no. bs-1178R; BIOSS), Occludin (1:100; cat. no. ab216327; Abcam), ZO-1 (1:300; cat. no. bs-1329-R; BIOSS) and TRPA1 (1:200; cat. no. ab58844; Abcam) at 4˚C overnight, followed by incubation with FITC-conjugated goat-anti-rabbit secondary antibodies (1:50; cat. no. bs-0295G-FITC; BIOSS) for 1 h at room temperature in the dark after washing in cold PBS.

Techniques: Expressing

Journal: Experimental and Therapeutic Medicine

Article Title: Effects of norepinephrine on colonic tight junction protein expression during heat stress

doi: 10.3892/etm.2021.9865

Figure Lengend Snippet: Expression levels of Occludin, ZO-1, PAR-2 and TRPA1 in Caco-2 cells after treatment with NE. (A) Representative western blotting images from four independent experiments. Comparison of the relative expression levels of (B) Occludin, (C) ZO-1, (D) PAR-2 and (E) TRPA1 in colon of control and NE-treated cells. * P<0.05 vs. Control. Data are presented as the mean ± SD. NE, norepinephrine; ZO-1, zonula occludens-1; PAR-2, protease activated receptor 2; TRPA1, transient receptor potential ankyrin 1 channel.

Article Snippet: The cells were then incubated with primary antibodies against PAR-2 (1:300; cat. no. bs-1178R; BIOSS), Occludin (1:100; cat. no. ab216327; Abcam), ZO-1 (1:300; cat. no. bs-1329-R; BIOSS) and TRPA1 (1:200; cat. no. ab58844; Abcam) at 4˚C overnight, followed by incubation with FITC-conjugated goat-anti-rabbit secondary antibodies (1:50; cat. no. bs-0295G-FITC; BIOSS) for 1 h at room temperature in the dark after washing in cold PBS.

Techniques: Expressing, Western Blot

Journal: Experimental and Therapeutic Medicine

Article Title: Effects of norepinephrine on colonic tight junction protein expression during heat stress

doi: 10.3892/etm.2021.9865

Figure Lengend Snippet: Immunofluorescence staining of Occludin, ZO-1, PAR-2 and TRPA1 in Caco-2 cells after 10 µM NE application. Staining of (A) Occludin, (B) ZO-1, (C) PAR-2 and (D) TRPA1. Magnification, x400. NE, norepinephrine; ZO-1, zonula occludens-1; PAR-2, protease-activated receptor 2; TRPA1, transient receptor potential ankyrin 1 channel.

Article Snippet: The cells were then incubated with primary antibodies against PAR-2 (1:300; cat. no. bs-1178R; BIOSS), Occludin (1:100; cat. no. ab216327; Abcam), ZO-1 (1:300; cat. no. bs-1329-R; BIOSS) and TRPA1 (1:200; cat. no. ab58844; Abcam) at 4˚C overnight, followed by incubation with FITC-conjugated goat-anti-rabbit secondary antibodies (1:50; cat. no. bs-0295G-FITC; BIOSS) for 1 h at room temperature in the dark after washing in cold PBS.

Techniques: Immunofluorescence, Staining